> For the complete documentation index, see [llms.txt](https://tk233.gitbook.io/notes/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://tk233.gitbook.io/notes/tools/using-the-leica-microscope-at-bwrc.md).

# Using the Leica Microscope at BWRC

## 1. Get Software

Log in to the computer.

<figure><img src="https://1287130752-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FvVJ0h2a4qMIhB1I8GdV8%2Fuploads%2Fgit-blob-f19c6aca9848e43abf148a5e87783c566a23e2a2%2Fimage.png?alt=media" alt=""><figcaption></figcaption></figure>

Open the "LAS V4.4" software.

<figure><img src="https://1287130752-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FvVJ0h2a4qMIhB1I8GdV8%2Fuploads%2Fgit-blob-be8ab616f773dfbdae99b996b11813031a4eec7d%2Fimage.png?alt=media" alt=""><figcaption></figcaption></figure>

If this window pops up, click "OK".

<figure><img src="https://1287130752-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FvVJ0h2a4qMIhB1I8GdV8%2Fuploads%2Fgit-blob-d8a83aca24d88e7c7eba6e9bc0b3f28e43cbed02%2Fimage.png?alt=media" alt=""><figcaption></figcaption></figure>

This is the main UI of the software.

<figure><img src="https://1287130752-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FvVJ0h2a4qMIhB1I8GdV8%2Fuploads%2Fgit-blob-36754aae85c49d16b7c410f4f9f15f68eb572933%2Fimage%20(1).png?alt=media" alt=""><figcaption></figcaption></figure>

## 2. Calibrate White Balance

Turn on the light of the microscope by pressing the power button.

<figure><img src="https://1287130752-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FvVJ0h2a4qMIhB1I8GdV8%2Fuploads%2Fgit-blob-c9afed449ecd46266cdf43a46e07b2ee9532d635%2Fimage.png?alt=media" alt=""><figcaption></figcaption></figure>

To do white balance calibration, put a white paper under the scope, and press this button on the software.

<figure><img src="https://1287130752-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FvVJ0h2a4qMIhB1I8GdV8%2Fuploads%2Fgit-blob-fbc08a9bc3bee6afdc9240aa20f512126ebfec14%2Fimage%20(2).png?alt=media" alt=""><figcaption></figcaption></figure>

## 3. Operating the Microscope

There are two knobs on the scope. The black one controls the focus length, and the white one controls the zoom.

<figure><img src="https://1287130752-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FvVJ0h2a4qMIhB1I8GdV8%2Fuploads%2Fgit-blob-2bd64ab405177285880088d00db5c18e7e06cfdc%2Fimage.png?alt=media" alt=""><figcaption></figcaption></figure>

## 4. Taking Photos

After framing the die in the center, click "Acquire Image (HDR)" button.

<figure><img src="https://1287130752-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FvVJ0h2a4qMIhB1I8GdV8%2Fuploads%2Fgit-blob-01938e674792286a9e296026fd3a9f13021d689f%2Fimage%20(3).png?alt=media" alt=""><figcaption></figcaption></figure>

The image will be saved at `C:\Users\Public\Documents\Leica Application Suite\Images\` by default.

<figure><img src="https://1287130752-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FvVJ0h2a4qMIhB1I8GdV8%2Fuploads%2Fgit-blob-8ab3270f1d9cbc3430609accdead10114621c7dd%2Fimage%20(4).png?alt=media" alt=""><figcaption></figcaption></figure>
